Generator

Part:BBa_K516222

Designed by: Federica Sampietro, Susanna Zucca, Daniele Sartori   Group: iGEM11_UNIPV-Pavia   (2011-09-08)

AiiA protein generator with Ptet and RBS B0032


aiiA lactonase protein generator driven by pTet promoter followed by BBa_B0032 medium efficiency RBS.
This is one of four systems with different RBSs provided to modulate the expression of AiiA lactonase enzyme (the other parts are: BBa_K516220 with BBa_B0030 RBS; BBa_K516221 with BBa_B0031 RBS ; BBa_K516224 with BBa_B0034 RBS).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 180
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aAiiA protein generator with Ptet and RBS B0032