Generator
Part:BBa_K516222
Designed by: Federica Sampietro, Susanna Zucca, Daniele Sartori Group: iGEM11_UNIPV-Pavia (2011-09-08)
AiiA protein generator with Ptet and RBS B0032
aiiA lactonase protein generator driven by pTet promoter followed by BBa_B0032 medium efficiency RBS.
This is one of four systems with different RBSs provided to modulate the expression of AiiA lactonase enzyme
(the other parts are: BBa_K516220 with BBa_B0030 RBS;
BBa_K516221 with BBa_B0031 RBS ;
BBa_K516224 with BBa_B0034 RBS).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 180
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
n/a | AiiA protein generator with Ptet and RBS B0032 |